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Proteintech anti c ebp homologous protein
Anti C Ebp Homologous Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+ebp+homologous+protein/CHOP%3B+GADD153+Antibody/pmc12940162-10-0-5
Average 96 stars, based on 856 article reviews

anti c ebp homologous protein - by Bioz Stars, 2026-11

96/100 stars

Images

Modification:

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol red-free DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue ® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM real-time PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1-AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts.
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1- AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

H2O2 Assay:

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts.
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1- AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

Reverse Transcription:

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts.
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1- AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

Real-time Polymerase Chain Reaction:

Phospho-proteomics:

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts.
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1- AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

Fluorescence:

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts.
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1- AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

Ubiquitin Proteomics:

Article Title: Eeyarestatin I (ESI)-induced ERAD inhibition exhibits anti-cancer activity through multiple mechanisms in human colorectal cancer cells.
Article Snippet: Endoplasmic reticulum (ER)-associated degradation (ERAD) is a cellular process for maintenance of protein homeostasis in the ER and aberration of ERAD regulation leads to abnormal function of ER.. As an inhibitory compound to ERAD, Eeyarestatin I (ESI) exhibits anti-cancer activity.. In this study, we elucidated the anti-cancer mechanisms of ESI-induced ERAD inhibition in human colorectal carcinoma cells.

other:

Article Title: BFGF alleviates diabetic endothelial dysfunction by downregulating Endoplasmic reticulum stress.
Article Snippet: vascular disease is the most common, 70-80% of diabetes patients die directly because of diabetes macrovascular complications [3].. Vascular endothelial dysfunction is the initial factor and main pathophysiological basis of vascular complications in diabetes, and also the early sign of diabetes vascular disease [4].. The ultimate goal of clinical treatment of diabetes is to prevent diabetes vascular disease and improve endothelial dysfunction.

Article Title: BFGF downregulates endoplasmic reticulum stress to alleviate HUVEC cell damage under high-glucose and inflammatory conditions.
Article Snippet: Primary antibodies to proliferating cell nuclear antigen (PCNA, 60097-1-Ig, 1:1000), B-cell lymphoma-2 (Bcl-2, 68103-1-Ig, 1:1000), Bcl-2-associated x (Bax, 50599-2-Ig, 1:1000), cleaved-caspase 3 (68773-1-Ig, 1:1000), activating transcription factor 4 (ATF4, 10835-1-AP, 1:1000), phospho-eukaryotic translation initiation factor 2 (p-eIF2, 68023-1-Ig, 1:1000), glucose-regulated protein 78 (GRP78, 66574-1-Ig, 1:1000), C/EBP-homologous protein (CHOP, 66741-1-Ig, 1:1000), and protein kinase RNA-like ER kinase (PERK, 68482-1-Ig, 1:1000) were purchased from the Proteintech group. β-actin (380624, 1:5000) was purchased from ZEN BIOP, platelet endothelial cell adhesion molecule-1 (CD31, sc-376764, 1:1000) was purchased from SANTA CRUZ, and vascular endothelial growth factor (VEGF, ab214424, 1:1000) was purchased from Abcam.

Polymerase Chain Reaction:

Article Title: A combination of ethanol and arachidonic acid promotes steatosis and endoplasmic reticulum stress and impairs mitochondrial respiration in H9c2 cardiomyoblasts.
Article Snippet: .. The reagents, chemicals, and supplies used in the current study includes: Dulbecco’s Modified Eagle’s Medium (DMEM, SH30243.01, Cytiva, MA, USA), phenol redfree DMEM (SH30284.01, Cytiva), XF DMEM medium (pH 7.4, 103575-100, Agilent Technologies, CA, USA), AA (U-71 A, Nu-Chek-Prep, Inc., MN, USA), ethanol (111000200, Greenfield Global, Canada), Triacsin C (10007448, Cayman Chemical, MI, USA), oil-red O powder (O0625, Sigma-Aldrich, MO, USA), Ponceau S solution (59803, Cell Signaling Technology, MA, USA), PrestoBlue® Cell Viability Reagent (Thermo Fisher Scientific, MA, USA), hydrogen peroxide assay kit (ab102500, Abcam, Cambridge, UK), TRIzol reagent (Ambion Life Technologies, MA, USA), NuGEN Universal Plus mRNA-Seq library kit (TECAN, CA, USA), 5× iScript reverse transcription supermix (1708841, Bio-Rad, CA, USA), iQ supermix (1708862, Bio-Rad), oligomycin (O4876, Sigma-Aldrich, MO, USA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, C2920, Sigma-Aldrich), rotenone (R8875, Sigma-Aldrich), antimycin A (A8674, Sigma-Aldrich), TaqmanTM realtime PCR primers (Applied Biosystems, MA, USA), including Atf4 (Rn00824644_g1), Ddit (Rn00492098_ g1), 18s (Applied Biosystems, 4352930E), antibodies against 4-Hydroxynonenal (4-HNE, ab46545, Abcam), glucose-regulated protein 78 (GRP78, 11587-1-AP, Proteintech, IL, USA), aldehyde dehydrogenases 1/2 (ALDH1/2, sc-166362, Santa Cruz Biotechnology, TX, USA), activating transcription factor 4 (ATF4, 10835-1- AP, Proteintech), C/EBP homologous protein (CHOP, 15204-1-AP, Proteintech), oxidative phosphorylation (OXPHOS) rodent antibody (45–8099, Thermo Fisher Scientific), anti-rabbit secondary antibody conjugated to horseradish peroxidase (7074, Cell Signaling Technology), anti-mouse StarBrightTM Blue 520 fluorescence secondary antibody (12005867, Bio-Rad), enhanced chemiluminescence reagent (1705062, Bio-Rad). .. H9c2 cell (CRL-1446TM), a rat cardio-myoblast cell line, was purchased from ATCC (VA, USA).

Expressing:

Article Title: Unlocking the potential of atractylenolide II: Mitigating non-alcoholic fatty liver disease through farnesoid X receptor-endoplasmic reticulum stress interplay
Article Snippet: .. The expression of specific proteins was detected using antibodies against ACTB (Cat# 3700, Cell Signaling Technology, Danvers, MA, USA), FXR (Cat# sc-25309, Santa Cruz Biotechnology, Santa Cruz, CA, USA), SERCA2 (Cat# A1097, ABclonal), small heterodimer partner (SHP; Cat# A1836, ABclonal), protein kinase, endoplasmic reticulum kinase (PERK; Cat# 20582-1-AP, Proteintech Group, Wuhan, China), phosphorylated PERK (p-PERK; Cat# 3179, Cell Signaling Technology), eukaryotic translation initiation factor 2 subunit α (eIF2α; Cat# 9722, Cell Signaling Technology), phosphorylated eIF2α (p-eIF2α; Cat# 3398, Cell Signaling Technology), glucose-regulated protein 78 (BIP; Cat# 3177, Cell Signaling Technology), activating transcription factor 4 (ATF4; Cat# 10835-1-AP, Proteintech Group), C/EBP homologous protein (CHOP; Cat# 15204-1-AP, Proteintech Group), AKT serine/threonine kinase (AKT; Cat# 9272, Cell Signaling Technology), phosphorylated AKT (p-AKT; Cat# 9271, Cell Signaling Technology), c-Jun N -terminal kinase (JNK; Cat# 66210-1-ig, Proteintech Group), phosphorylated JNK (p-JNK; Cat# 4668, Cell Signaling Technology) and Histone H3 (H3; Cat# 4499, Cell Signaling Technology). .. Protein blots were visualized using Chemi-Lumin One Ultra (Tanon Science & Technology Co., Ltd., Shanghai, China) with a chemiluminescence detection kit (Cat# 34577, Thermo Fisher Scientific).

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